Use the sterile Decron swab to collect secretions on the vaginal surface.Open the swab bag and place the swab into the vagina(5 cm depth) for 1 minute.
Alternatively, speculum may be used and vaginal secretion may be collected by leaving the swab into the vagina(5 cm depth) for 15 seconds.
Step One
Bring the complete kit and samples to be tested to room temperature prior to testing. Open the unit dose vial and lay it vertically on a flat and horizontal surface.Dip the Swap into the unit dose vial and rotate for 10 seconds.
Step Two
2Dip the strip into the vial with arrows pointing downwards. Gently hit dipstick on the bottom of the tube to enhance migration. Keep the strip in the tube in vertical position for 10 minutes.
Step Three
Read the result after 10 minutes from the time the strip is dipped in the tube.Donot interpret any test band appearing 15 minutes after the strip is dipped into the vial.
Principle
The premature rupture of membranes or PROM is relatively frequent and concerns 5 to 10 % of pregnancy cases. It might lead to preterm delivery and fetal infection. The leakage of amniotic liquid is not always detectable by conventional clinical examination and confirmatory biological test is sometimes useful. Biological tests are based on vaginal detection of alkalinization (easy to proceed, sensitive,inexpensive but poor specific) or presence of a molecule which is physiologically present in high concentration in amniotic fluid(diamine oxidase , alpha feto protein, fibronectin, IGFBP-1).
Amnioquick is based on detection of IGFBP-1 which is found in very high concentration in amniotic fluid.Amnioquick can detect less than 1l of amniotic fluid from the collection swab.The test can be used for detection of microrupture or frank rupture of the fetal membranes.
Note: In near term women, positive results may appear even in absence of rupture of membranes as the decidual cells of the cervix may release phosphorylated IGFBP-1 that cross react with the test.
Interpretation
POSITIVE Presence of a purple test line(even if intensity is weak) and a purple control line.
Presence of amniotic fluid in sample.
NEGATIVE Absence of purple test line and the presence of a purple control line.Amniotic fluid is not present in sample.
INVALID A wrong procedure and high viscocity of sample are the two most frequent causes of invalid results. The test has to be run again with a new strip.
